5-Me-UTP 100mM Solution Amount/Package:1mL / centrifuge tube

5-Me-UTP 100mM Solution Amount/Package:1mL / centrifuge tube5 methyluridine triphosphate (5mUTP) is a chemically modified UTP analog that supports production of methyl modified RNAs with reduced immunogenicity and improved stability. This 100 mM disodium salt solution provides 5mUTP in a highly soluble, ready to use form suitable for both routine laboratory applications and high consistency manufacturing environments. Applications Modified RNA Synthesis In vitro transcription of chemically modified mRNA with

Heating the solution of RNA prior to incubation with Vaccinia capping enzyme

exonuclease and RNase activities

Manufactured to ≥98% purity with tightly controlled impurity specifications

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sequencing primers

RNA cyclisation: circular RNA is produced by joining the two ends within the RNA molecule by T4 RNA ligase 1 (T4 Rnl1) or T4 RNA ligase 2 (T4 Rnl2) catalysing the joining of 5'-phosphate and 3'-hydroxyl groups at the RNA terminus

" Proceedings of the National Academy of Sciences 97

Its molecular formula is 3'-OMe-m7G(5')ppp(5')G

Concentration: 1U/μL

Minimized GTP competition

mix well and incubate 30min at 37°C

3 (2023): e202200169